How To Peroxide (H2O2) dosing for parasites in reef tank

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clhardy5

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Looking at your Anthias posts I would 100% say you had Ich. So this is really nice to see you have a negative test come back. I assume you did 3x a day + every 15min 12am-6am?I
I did the dosing schedule exactly as Jessican did :). The Anthias actually never made it into the tank.....All 4 that I bought died during QT :-( Look for the pictures of my brown tang...I think I posted a link to that post earlier in this thread.
 

JMP1220

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Hello, looking for a little bit of advice with dosing H2o2 to eradicate Uronema. A recent Aquabiomics test found Uronema in our display tank (to our surprise). We've never actually had an outbreak and we have never seen any signs of it before. We also don't have any Chromies or Anthias, but we do have clownfish which I know are in the Damsel family (not sure how succeptible to Uronema they are).

Since then we have been dosing Peroxide 3x daily at the recommended full dose for about 4 to 6 weeks so far but have not been doing the overnight doses yet. We also have a UV sterilizer running but the flow rate is set high to target algae/bacteria as opposed to parasites (however I would imagine this will still enhance the effectiveness of the peroxide but not sure on this).

My questions are, how necessary are the overnight doses to eradate Uronema if I've never had an outbreak, and second, since Uronema was deteced, should I be feeding pharma grade Metronidazole (edit: via food soaking) for a couple weeks? Thank you very much in advance!
 
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anthonys_aqua

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@Jessican would you say you lost a lot of bacteria due to the peroxide dosing? I am noticing diatoms even though my tank is 10 months old which I assume so peroxide is killing bacteria as well?

Scratch that, I guess I got dinos now
 
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Jessican

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My questions are, how necessary are the overnight doses to eradate Uronema if I've never had an outbreak, and second, since Uronema was deteced, should I be feeding pharma grade Metronidazole (edit: via food soaking) for a couple weeks? Thank you very much in advance!
I feel that the overnight dosing is the most important bit, since it keeps a consistent, low level of peroxide in the system for multiple hours.

Feeding metro sounds like a good idea to me, too.

@Jessican would you say you lost a lot of bacteria due to the peroxide dosing? I am noticing diatoms even though my tank is 10 months old which I assume so peroxide is killing bacteria as well?

Scratch that, I guess I got dinos now
I do think that the peroxide can negatively affect the beneficial bacteria, especially at higher levels of dosing. I perodically added bottled bacteria to try to help with this.

Did you confirm dinos under a microscope? I thought I had dinos at one point during dosing, and it turned out to be euglena instead, which I was able to get rid of with manual removal.
 

bishoptf

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So I finally have my doser ready to go but have a question about dosing containers, I usually use clear containers so I can see levels etc but I thought h202 would break down in light but maybe that is a long process. I can use the brown bottles that it comes in but was wondering what folks were doing and if the short period of time exposed to light in a clear container.

Sorry another dumb question, lol....

Here is my dry runs for my dosing set up:

doser-h202.png
 

bishoptf

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Keep it in the bottles, and also use opaque tubing (I used RODI tubing). We’ve seen issues with bubbles forming in the lines otherwise.
I have some black airline tubing so I will use that and see how it goes and just top off the bottles every few days, fun fun... :)
 

bryanr

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My first Aquabiomics DNA test reported almost 97% of the DNA was ich. Aquabiomics even said that was the highest reading they had ever gotten on a test. Well got my 2nd test back today, I dosed H2O2 for almost 90 days, but the ich percent is 0. I do have a parasite that infects mollusks, but the ich is gone. I will continue dosing until I get some fish through quarantine and find out if Perkinsus can cause any issues in fish.

Parasites​


TypePercent of eDNAGroupName
sq11070.113Perkinsusuncultured_marine
View the full table
GroupDescriptionPercent of eDNA
AmyloodiniumDinoflagellate parasites responsible for the fish disease Marine Velvet0
CryptocaryonCiliate parasites responsible for the fish disease Ich0
BrooklynellaCiliate parasites responsible for the fish disease Brook0
UronemaCiliate parasites responsible for a fish disease called by the same name0
MiamiensisCiliate parasites responsible for a fish disease called Uronema or Scuticociliatosis0
PhestillaMontipora-eating Nudibranchs0
AmakusaplanaAcropora-eating Flatworms0
ProsthiostomumAcropora-eating Flatworms0
AncyrocephalidaeFlatworms often called Flukes that parasitize fish0
GyrodactylidaeFlatworms often called Flukes that parasitize fish0
CapsalidaeFlatworms often called Flukes that parasitize fish0
TurbonillaSnails in the family Pyramidellidae that parasitize giant clams0
PerkinsusApicomplexan parasites of clams and other bivalves0.113
TrichodinaA ciliated protozoan parasite of fish0
 

GGG

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Thanks for this great information Jessica

I plan to do a H2O2 bath for some new fish however I too dumb to work out the dose...

"For reference, using 3% peroxide:
1mL/10gal (40L) = 0.75ppm
1mL/8gal (30L) = 0.9ppm
1mL/5gal (19L) = 1.59ppm
1mL/3gal (11L) = 2.73ppm
1mL/1gal (4L) = 7.5ppm"

How many mL per g (or L) = 150ppm?

Thanks in advance.
 

Jessican

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Thanks for this great information Jessica

I plan to do a H2O2 bath for some new fish however I too dumb to work out the dose...

"For reference, using 3% peroxide:
1mL/10gal (40L) = 0.75ppm
1mL/8gal (30L) = 0.9ppm
1mL/5gal (19L) = 1.59ppm
1mL/3gal (11L) = 2.73ppm
1mL/1gal (4L) = 7.5ppm"

How many mL per g (or L) = 150ppm?

Thanks in advance.
So what I’m doing here is in-tank dosing, not a bath, so much smaller dosages. What you want is here: Hydrogen Peroxide :)

From that link:
Dosing instructions: To achieve ~ 150 ppm H2O2 add:
  • 1.25 ml of 3% H2O2 per 1 cup of saltwater.
OR
  • 5 ml of 3% H2O2 per 1 liter of saltwater.
OR
  • 20 ml of 3% H2O2 per 1 gallon of saltwater.
 
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Fred949

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so i finally got my DNA test back and no ich or flukes but some other parasites, anyone know if I should be worried and if H202 has any effect on them? I have been dosing for 1 month now and no other fish losses and they seem to be looking healthy.

1660733409991.png
 
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Jessican

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So I don’t personally know anything about Perkinsus, but that just showed up in @bryanr’s tests, too, and apparently it only affects bivalves, like clams.

We have seen success with peroxide eliminating Uronema heteromarinum, @smsreef had it in his tank and was able to clear it with dosing.
 

bishoptf

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Anyone have dosed h202 while flucanazole was in the tank? I have been treating the tank for several weeks with Fluc to kill some bryopsis and wanted to start my h202 but was just curious if anyone had done it in the past, I do not think it should be an issue but thought I would ask.

Thanks
 

Jessican

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Anyone have dosed h202 while flucanazole was in the tank? I have been treating the tank for several weeks with Fluc to kill some bryopsis and wanted to start my h202 but was just curious if anyone had done it in the past, I do not think it should be an issue but thought I would ask.

Thanks
Don’t mix peroxide with any other medications. At best, one can render the other ineffective; at worst, one can make the other toxic.
 

bishoptf

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Don’t mix peroxide with any other medications. At best, one can render the other ineffective; at worst, one can make the other toxic.
Ok well I guess I continue to wait until I get all the bryopsis cleaned up, was hoping that it wouldn't be an issue, thanks.
 

NPA

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My first Aquabiomics DNA test reported almost 97% of the DNA was ich. Aquabiomics even said that was the highest reading they had ever gotten on a test. Well got my 2nd test back today, I dosed H2O2 for almost 90 days, but the ich percent is 0. I do have a parasite that infects mollusks, but the ich is gone. I will continue dosing until I get some fish through quarantine and find out if Perkinsus can cause any issues in fish.

So I don’t personally know anything about Perkinsus, but that just showed up in @bryanr’s tests, too, and apparently it only affects bivalves, like clams.

We have seen success with peroxide eliminating Uronema heteromarinum, @smsreef had it in his tank and was able to clear it with dosing.

Perkinsus should not be a concern for fish, it is believed to be the cause of Pinched Mantle Disease, a prominent killer of Tridacna clams though, so much so that I doubt you'd be able to keep any in a system that it was detected in.
 

NPA

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Got my pre-UV/H2O2 test results back as well:

1660753554517.png


This sample was taken in early July after I lost a handful of fish to what I'm assuming was the Uronema based on the symptoms to death speed, and I've been running UV with H2O2 (overnight dosing only) for 30 days and fish have been symptom free for ~ 3 weeks. All of the surviving fish did get an API general cure + focus food treatment for a week concurrent with dosing.

I'll probably send off a second sample in early September now that AB looks like they're doing twice monthly DNA tests rather than just EoM as was the case with my first sample.
 

sihaya

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First, I want to thank the OP for sharing the results of his experiments. The post inspired me to do some reading and I have a theory on why dosing hydrogen peroxide seems to help manage these diseases.

When mixed in salt water, hydrogen peroxide releases oxygen and ozone. It’s this fact that inspired the invention of a device called a Schoting Oxydator. This thing slowly releases Hydrogen Peroxide from a chamber. It’s quite genius and worth a Google search.

A recent study on the effectiveness of ozone and UV on c. Irritans shows that ozone can be very effective for killing the parasite in multiple stages of life. This might explain why dosing hydrogen peroxide helps.
 

Colin_S

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I'm 6 days into a 42day dosing program. Everything seems ok, corals sulking a little but fish eating well and Clown fish even spawned.
I went for this routine.
water volume 140g
daytime 84ml = 28ml per dose, starting at 06:00, 14:00 & 22:00
evening 168ml = 14ml per dose, starting at 23:30 then every 30mins for 12 doses

I hope this is correct..
 

NPA

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I'm 6 days into a 42day dosing program. Everything seems ok, corals sulking a little but fish eating well and Clown fish even spawned.
I went for this routine.
water volume 140g
daytime 84ml = 28ml per dose, starting at 06:00, 14:00 & 22:00
evening 168ml = 14ml per dose, starting at 23:30 then every 30mins for 12 doses

I hope this is correct..

Your overnight dose is too high. You want 2x a single daytime dose for the overnight, in your case 56ml overnight spread over 6 hours.
 
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